Measurement

Part:BBa_K4630401:Design

Designed by: Lei Bai   Group: iGEM23_WHU-China   (2023-10-08)


EGFP inserted with 50*( N20+PAM)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 993
    Illegal SpeI site found at 569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1092
    Illegal NheI site found at 1603
    Illegal NheI site found at 1613
    Illegal NheI site found at 1672
    Illegal SpeI site found at 569
    Illegal NotI site found at 140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 993
    Illegal SpeI site found at 569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 993
    Illegal SpeI site found at 569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 1354


Design Notes

The N20s sequence is not easy to synthesize.


Source

EGFP was cloned from pET28a-EGFP and the N20s sequence was synthesized.

References